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Predef 2X (Isoflupredone acetate) Dose for Cattle & Horses: Strengths & Dose Calculator

Q: What is the Predef 2X dose for cows with ketosis?

10–20 mg IM (5–10 mL), repeatable in 12–24 hours.

Reviewed by Dr. Ravi Baraiya, B.V.Sc.Updated 2 Nov 20262 sources

Predef 2X is isoflupredone acetate 2 mg/mL from Zoetis, used for primary bovine ketosis and inflammatory conditions in cattle, horses and pigs ^[1]. Cattle get 10–20 mg IM, horses 5–20 mg and pigs 5 mg for a 300 lb animal ^[1].

Contains: Isoflupredone acetate
Company: Zoetis
Forms: Predef 2X injection 2 mg/mL
Label species: Cattle, Horses, Pigs
Data from: USA (FDA label)
Type: Corticosteroid

Predef 2X dose calculator

Results are calculated in your browser from the doses and strengths on this page. Check the strength printed on your pack and the label in your country before giving any dose.

Predef 2X strengths and presentations

Presentation	Contains	Strength check
Predef 2X injection 2 mg/mL IM / intrasynovial	Isoflupredone acetate 2 mg per mL^[1]	Confirmed

Predef 2X dose by species

Species	Use	Dose	Route	How often	Basis
Cattle	Ketosis and inflammatory conditions	10–20 mg/animal^[1]	IM	May be repeated in 12–24 hours	Label
Horses	Inflammatory conditions	5–20 mg/animal^[1]	IM	Repeated as necessary	Label
Pigs	Inflammatory conditions 5 mg for a 300 lb pig.	5 mg/animal^[1]	IM		Label

Label directions

Cattle: 10–20 mg IM by size and severity; may repeat in 12–24 hours ^[1].
Horses: 5–20 mg IM, repeated as necessary ^[1].
Pigs: 5 mg IM for a 300 lb animal ^[1].

Warnings and precautions

Stop if signs of low potassium (hypokalaemia) appear ^[1].
May induce parturition in the last trimester of pregnancy ^[1].
Do not use in veal calves or in horses intended for human consumption ^[1].

Withdrawal periods

Species	Product	Withdrawal	Detail
Cattle	Meat	7 days	Not slaughtered within 7 days of the last treatment.^[1]
Pigs	Meat	7 days	Not slaughtered within 7 days of the last treatment.^[1]

About isoflupredone acetate (the active ingredient)

Corticosteroid (glucocorticoid with mineralocorticoid activity).

How it works

Glucocorticoids bind receptors in the cell cytoplasm and the hormone–receptor complex changes which proteins the cell makes; one of these, lipocortin, inhibits phospholipase A2, the first step of prostaglandin and leukotriene production ^[2]. Isoflupredone lacks selectivity for glucocorticoid over mineralocorticoid effects ^[2].

Key safety points

Increases the risk of severe hypokalaemia — stop treatment if signs appear ^[2]^[1].
May induce the first stage of parturition in the last trimester of pregnancy ^[1].
Do not use in calves to be processed for veal or in horses intended for human consumption ^[1].

All about isoflupredone acetate — doses by species and every brand →

Pack sizes

100 mL vials ^[1].

Frequently asked questions

What is the Predef 2X dose for cows with ketosis?

10–20 mg IM (5–10 mL), repeatable in 12–24 hours ^[1].

What is the meat withdrawal for Predef 2X?

7 days ^[1].

More from Zoetis

All veterinary products →

Predef 2X is a trademark of its owner. This page is an independent clinical reference and is not affiliated with Zoetis. Strengths, species and withdrawal periods can differ between countries — always follow the pack insert in your country.

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Medically reviewed by

Dr. Ravi Baraiya, B.V.Sc.

Veterinarian · Reviewed 2 Nov 2026

References

PREDEF 2X (isoflupredone acetate) — labelDrugs.com Vet — US label text, Zoetis · 2026 · Product label
Passage read from this source
Each mL of PREDEF 2X contains 2 mg of isoflupredone acetate; also 4.5 mg sodium citrate hydrous;
is an effective and valuable treatment for the endocrine and metabolic imbalance of primary bovine ketosis.
The usual intramuscular dose for cattle is 10 to 20 mg, according to the size of the animal and severity of the condition.
This dose may be repeated in 12 to 24 hours if indicated.
Corticosteroids in AnimalsMerck Veterinary Manual · 2024 · Veterinary reference
Passage read from this source
Glucocorticoid-specific receptors in the cell cytoplasm bind with steroid ligands to form hormone-receptor complexes
Such proteins include lipocortin, a protein known to inhibit PLA2alpha
but lacks selectivity for mineralocorticoid effects and increases the risk of severe hypokalemia.

For veterinary professionals. Information supports — never replaces — clinical judgement; always check the current product label and local regulations.